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Diagnosis of group A coxsackieviral infection using polymerase chain reaction

¹ Department of Pediatrics, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan
² Infectious Disease Laboratory, Mitsubishi Kagaku Bio-Clinical Laboratories, Inc., Tokyo 174-8555, Japan
³ Fukushima Institute for Public Health and Environmental Science, Fukushima 960-8560, Japan

Correspondence to:
Correspondence to:
Dr M Hosoya, Dept of Pediatrics, Fukushima Medical University School of Medicine, Hikarigaoka 1, Fukushima 960-1295, Japan;
mhosoya{at}fmu.ac.jp

Aims: To examine the relation between enteroviral infection, especially group A coxsackieviral infection, and acute febrile illness over two summers using tissue culture and polymerase chain reaction (PCR).

Methods: Throat swabs were collected from 246 children from June to August 1997 and 1998.

Results: Enteroviruses were isolated from 33/246 samples and 35 other viruses were isolated. Enteroviral genomes were detected in 54/178 samples from which no virus was isolated. Of 41 enteroviral genotypes identified by sequence analysis of PCR products, 38 were group A coxsackieviruses, which are usually difficult to isolate using tissue culture.

Conclusion: Results indicate that viral detection and identification based on PCR is useful in the diagnosis of group A coxsackieviral infection.

Keywords: coxsackievirus group A; herpangina; polymerase chain reaction; phylogenetic

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