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Arch Dis Child 1997;77:148-149 ( August )

Eubacterial approach to the diagnosis of bacterial infection

B E Ley,a C J Linton,b S Longhurst,a H Jalal,b M R Millarb

a Bristol Royal Hospital for Sick Children, Bristol, b Department of Pathology and Microbiology, University of Bristol, Bristol

Correspondence to: Dr B E Ley, Department of Paediatric Oncology, Bristol Royal Hospital for Sick Children, St Michael's Hill, Bristol BS2 8JS.

Accepted 29 April 1997

Computed tomography revealed a frontal empyema in an 8 month old boy who presented with a prolonged convulsion. Before admission he had received oral antibiotics and all specimens were negative on culture. The presence of bacteria in cerebrospinal and empyema fluid was demonstrated using the polymerase chain reaction (PCR) with 16S rRNA gene primers. A presumptive identification of Streptococcus pneumoniae was made by comparison of PCR products with those derived from known bacteria using denaturing gradient gel electrophoresis.

Keywords: meningitis; Streptococcus pneumoniae; polymerase chain reaction

© 1997 by Archives of Disease in Childhood


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